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• Taking Coursework to Better Understand the Integration of Soil, Microbiology, Hydrology, and Chemistry, and How This Pertains to Soil Health. Knowledge Gained will be Utilized on MY Farm to Improrve Soil Health, Increase Crop Yields, and Leave the Land in Better Shape Than When Acquired.
• Dissertation Title: Characterization of Vpa0450, A Type iii Secreted Effector Protein from Vibrio Parahaemolyticus. I Utilized Various Molecular, Cell and Microbiology Techniques to Characterized the Contribution of A Type iii Secreted Effector to the Pathogenesis of Vibrio Infection. i was Able to Adapt Genetic Techniques for Genome Manipulation Developed for Other Organisms to Our System, Allowing for Multiple Gene Deletions and Better Characterization of the Pathogenicity Determinants of This Organism. Various Mutants of Vibrio were Used to Infect Cultured Cells with Downstream Analysis by Confocal Microscopy, Western Blotting, and Biochemical Analysis
• i Worked on A Qualitative Proteomic Analysis of A Short-Chained Alkene Degrading Bacteria. the Goal was to Identify the Biosynthetic Enzymes Used to Make Coenzyme M, A Metabolic Cofactor Used in the Catalytic Reduction of an Epoxide Intermediate in Propylene Metabolism. Peptides were Separated Using Offline Scx Followed by Online C18 Rp. Detection was done with A Thermo Lcq Advantage MS Equipped with A Picoview Nanospray Source. Title of Thesis: Proteomic Identification of Putative Coenzyme M Biosynthetic Genes Expressed by Xanthobacter Autotrophicus Py2