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• GH Gene Expression is Regulated by the Pituitary Specific Transcription Factor Pit-1 (Aka Pou1f1). Several Isoforms of This Factor are Expressed in the Anterior Pituitary, but Which of Them are Involved in GH Gene Expression was not Known. MY Work Identified A Novel Pit-1 Isoform, the Pit1β2. i Demonstrated That All Isoforms Except the Pit1γ could Activate the Promoter and That All the Isoforms Localized to the Nucleus. Using Gel-Shift Assay i Showed That the Pi1γ Isoform did not Bind The… Show More GH Gene Expression is Regulated by the Pituitary Specific Transcription Factor Pit-1 (Aka Pou1f1). Several Isoforms of This Factor are Expressed in the Anterior Pituitary, but Which of Them are Involved in GH Gene Expression was not Known. MY Work Identified A Novel Pit-1 Isoform, the Pit1β2. i Demonstrated That All Isoforms Except the Pit1γ could Activate the Promoter and That All the Isoforms Localized to the Nucleus. Using Gel-Shift Assay i Showed That the Pi1γ Isoform did not Bind the Proximal Pit1 Binding Site as Well as the Other Isoforms. i Found A Functional Advantage for Having Multiple Isoforms Since Expression of More Than Isoform from Recombinant Plasmids Activated the Cgh Promoter Synergistically. Show Less